Madhya Pradesh Board Biotechnology Syllabus for Class 11

Madhya Pradesh Board Syllabus for Class 11 Biotechnology

Bio-Technology syllabus for class XI of 2008-09 session

Time: 3 hours
Maximum Marks: 100 ( Theory-75 Marks, Practical-25 Marks)

Unit-I	Introduction To Biotechnology	Fundamentals of biochemical engineering Biotechnology and society.	10 Marks
Unit-II	Bio-molecules	Building blocks of Bio-molecules and dynamic structure and functions of macromolecules, Biochemical Techniques.	20 Marks
Unit-III	Cell and Development	The basic unit of life. Cell growth and development Cellular Techniques	20 Marks
Unit-IV	Genetics and Molecular Biology	Principles of genetics Genome functions Genetic Techniques	25 Marks

Practical Syllabus:-

Note:- Every student is required to do the following Experiments during the academic session

List of Experiments:-

1. Preparation of buffers and PH determination.

2. Sterilization techniques (wet and dry sterilization, chemical sterilization and ultra filtration.)

3. Media preparation (Solid and liquid LB Medium)

4. Isolation of Bacteria from curd and staining of Bacteria.

5. Determination of Bacterial growth curve.

6. Study of various stages of mitosis and calculation of mitotic index.

7. Preparation of Karyotype.

8. Cell Viability assay (using Evans blue stain)

9. Cell counting (using Haemocytometer).

10.Determination of blood groups.

11.Isolation of genomic DNA.

12.Detection of DNA by Gel Electrophoresis.

13.Estimation of DNA.

14.Isolation of milk protein. (casein)

15.Estimation of protein by Biuret method.

16.Assessing the enzyme acid phosphate.

5 Plant Cell Culture and Applications -10 Marks

- Introduction

- Cell and Tissue Culture Techniques.

- Application of Cell and Tissue Culture.

- Gene Transfer Methods in Plants.

- Transgenic Plant with Beneficial-Traits.

- Diagnostics in Agriculture and Molecular Breeding.

- Bioethics in Plant Genetic Engineering

6 Animal Cell Culture and Applications -10 Marks

- Introduction

- Animal Cell-Culture Techniques.

- Characterization of Cell Lines.

- Scale up of Animal Culture Process.

- Applications of Animal Cell Culture.

- Stem Cell Technology.

- Bioethics of Genetic Engineering in Animals.

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