Gujarat Board Biotechnology Syllabus for Class 12

CLASS XII
(THEORY)
One paper                      Time: 3Hours                Total Marks : 70

Unit 1: Protein and Gene Manipulation                             Marks 40

Chapter I: Protein Structure and Engineering                  15 Marks
-Introduction to the world of Proteins
-3-D Shape of Proteins
-Structure Function relationship in Proteins
-Purification of Proteins
-Characterization of Proteins
-Protein based products
-Designing Proteins
-Proteomics

Chapter II: Recombinant DNA Technology 15 Marks

-Introduction
-Tools of rDNA Technology
-Making Recombinant DNA
-DNA Library
-Introduction of Recombinant DNA into host cells
-Identification of recombinants
-Polymerase Chains Reaction (PCR)
-DNA Probes
-Hybridization Techniques
-DNA Sequencing
-Site-directed mutagenesis

Chapter III: Genomics and Bioinformatics 10 Marks

-Introduction
-Genome Sequencing Projects
-Gene Prodiction and counting
-Genome similarity, SNP's and comparative genomics
-Functional Genomics
-History of Bioinformatics
-Sequences and Nomenclature
-Information Sources
-Analysis using Bioinformatics tools.

Unit II : Cell Culture Technology                                             10 Marks

Chapter I: Microbial Culture and Applications

-Introduction
-Microbial Culture Techniques
-Measurement and Kinetics of microbial Growth
-Scale up of microbial process
-Isolation of microbial products
-Strain isolation and Improvement
-Applications of microbial culture technology
-Bio safety of genetically modified micribes

Chapter II: Plant Cell Culture and Applications 10 Marks

-Introduction
-Cell and Tissue Culture Techniques
-Applications of Cell and Tissue Culture
-Gene Transfer Methods in Plants
-Transgenic Plants with Beneficial Traits
-Diagnostics in Agriculture and Molecular Breeding
-Bio safety of genetically modified plants

Chapter III: Animal Cell Culture and Applications 10 Marks

-Introduction
-Animal Cell Culture Techniques
-Characterisation of Cell Lines
-Scale-up of Animal Culture Process
-Applicationsof Animal Cell Culture
-Stem Cell Technology
-Bio safety of genetically modified animals

Practical's

Note: Every student will be required to do the following experiments during the academic session
List of Experiments

1. Isolation of bacterial plasmid DNA and its detection by gel eletrophoresis

2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis

3. Bacterial transformation using any plasmid

4. Data retrival and data base search using internet site NCBI

5. Download a DNA and protein sequence from internet, analyse and comment on it.

6. Cell viability assay (using Evans blue Stain)

7. Determination of blood groups.

8. Estimation of DNA

9. Ion-exchange chromatography for proteins.

10. Reading of a DNA sequencing gel and arrive at the sequence.

11. Estimation of blood glucuse by enzymatic method (GOD/POD)

12. Project work.