CBSE Board Biotechnology Syllabus for Class 12

                                                                  CLASS XII
(THEORY)

One paper

Unit V: Protein and Gene Manipulation

Chapter I: Recombinant DNA Technology
Introduction
Tool of rDNA Technology
Marketing Recombinant DNA
Introduction of Recombinant DNA into Host Cells
Identification of Recombinants
DNA Library
DNA Probes
Hybridization Techniques
Polymerase Chain Reaction (PCR)
DNA Secquencing
Site-directed Mutagenesis

Chapter II: Genomics and Bioinformatics
Introduction
Genome Sequencing Projects
Gene Prediction and Counting
Genome Similarity, SNPs and Comparative Genomics
Functional Genomics
Proteomics
History of Bioinformatics
Sequences and Nomenclature
Information Sources
Analysis using Bioinformatics Tools

Chapter III: Proteing Structure and Engineering
Introduction to the World of Proteins
3-D Shape of Proteins
Structure-Function Relationship in Proteins
Purification of Proteins
Characterization of Proteins
Protein based Products
Designing Proteins

Unit VI : Cell Culture Technology

Chapter I: Microbial Culture and Applications
Introduction
Microbial Culture Techniques
Measurement and Kinetics of microbial Growth
Scale up of microbial process
Isolation of microbial products
Strain isolation and Improvement
Applications of microbial culture technology
Biosafety of genetically modified microbes

Chapter II: Plant Cell Culture and Applications
Introduction
Cell and Tissue Culture Techniques
Applications of Cell and Tissue Culture
Gene Transfer Methods in Plants
Transgenic Plants with Beneficial Traits
Biosafety in Plant Genetic Engineering

Chapter III: Animal Cell Culture and Applications
Introduction
Animal Cell Culture Techniques
Characterisation of Cell Lines
Scale-up of Animal Culture Process
Applications of Animal Cell Culture
Stem Cell Technology
Bio safety of genetically modified animals

Practicals

Note: Every student will be required to do the following experiments during the academic
Session

List of Experiments
1. Isolation of bacterial plasmid DNA and its detection by gel electrophoresis
2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
3. Bacterial transformation using any plasmid
4. Data retreival and data base search using internet site NCBI
5. Download a DNA and protein sequence from internet and analyse.
6. Cell viability assay
7. Determination of blood groups.
8. Estimation of DNA
9. Ion-exchange chromatography of proteins.
10. Reading DNA sequence.
11. Estimation of blood glucose by enzymatic method (GOD/POD)
12. Project work.

Scheme of Evaluation:
The scheme of evaluation at the end of the session will be as under:
A. Two experiments
Practical record
Viva on Practicals
B. Project work
Write up
Viva on project