CBSE Board Biotechnology Syllabus for Class 12
CBSE Board Syllabus for Class 12 Biotechnology
CLASS XII
(THEORY)
One paper
Unit V: Protein and Gene Manipulation
Chapter I: Recombinant DNA Technology
Introduction
Tool of rDNA Technology
Marketing Recombinant DNA
Introduction of Recombinant DNA into Host Cells
Identification of Recombinants
DNA Library
DNA Probes
Hybridization Techniques
Polymerase Chain Reaction (PCR)
DNA Secquencing
Site-directed Mutagenesis
Chapter II: Genomics and Bioinformatics
Introduction
Genome Sequencing Projects
Gene Prediction and Counting
Genome Similarity, SNPs and Comparative Genomics
Functional Genomics
Proteomics
History of Bioinformatics
Sequences and Nomenclature
Information Sources
Analysis using Bioinformatics Tools
Chapter III: Proteing Structure and Engineering
Introduction to the World of Proteins
3-D Shape of Proteins
Structure-Function Relationship in Proteins
Purification of Proteins
Characterization of Proteins
Protein based Products
Designing Proteins
Unit VI : Cell Culture Technology
Chapter I: Microbial Culture and Applications
Introduction
Microbial Culture Techniques
Measurement and Kinetics of microbial Growth
Scale up of microbial process
Isolation of microbial products
Strain isolation and Improvement
Applications of microbial culture technology
Biosafety of genetically modified microbes
Chapter II: Plant Cell Culture and Applications
Introduction
Cell and Tissue Culture Techniques
Applications of Cell and Tissue Culture
Gene Transfer Methods in Plants
Transgenic Plants with Beneficial Traits
Biosafety in Plant Genetic Engineering
Chapter III: Animal Cell Culture and Applications
Introduction
Animal Cell Culture Techniques
Characterisation of Cell Lines
Scale-up of Animal Culture Process
Applications of Animal Cell Culture
Stem Cell Technology
Bio safety of genetically modified animals
Practicals
Note: Every student will be required to do the following experiments during the academic
Session
List of Experiments
1. Isolation of bacterial plasmid DNA and its detection by gel electrophoresis
2. Restriction digestion of plasmid DNA and its analysis by gel electrophoresis
3. Bacterial transformation using any plasmid
4. Data retreival and data base search using internet site NCBI
5. Download a DNA and protein sequence from internet and analyse.
6. Cell viability assay
7. Determination of blood groups.
8. Estimation of DNA
9. Ion-exchange chromatography of proteins.
10. Reading DNA sequence.
11. Estimation of blood glucose by enzymatic method (GOD/POD)
12. Project work.
Scheme of Evaluation:
The scheme of evaluation at the end of the session will be as under:
A. Two experiments
Practical record
Viva on Practicals
B. Project work
Write up
Viva on project
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Board Sample Paper
- CBSE Board Class 12 Computer Science 2008
- Tamilnadu Board Class 10 Science Sample Paper Of 2011
- Himachal Pradesh Board Class 11 Physical Education 2008
- CBSE Board Class 11 Political Science 2008
- ICSE Board Class 10 Home Science 2010
- Madhya Pradesh Board Class 12 Crop Production 2013-SET-3
- CBSE Board Class 10 Physics 2010
- West Bengal Board Class 12 Chemistry Sample Paper Of 2011
- Bihar Board Class 12 2012
- CBSE Board Class 11 Physics 2011
Previous Year Paper
- CBSE Board Class 11 Economics 2011
- CBSE Board Class 10 English Core 2007
- CBSE Board Class 12 Business Studies 2009
- CBSE Board Class 12 Computer Science 2010
- CBSE Board Class 12th Biology Previous Year Question Paper 2007
- CBSE Board Class 10 Science 2010
- CBSE Board Class 12 Physics 2010
- CBSE Board Class 12 Home Science 2007
- CBSE Board Class 12 Psychology 2007
- CBSE Board Class 11 Chemistry 2007
Syllabus
- Manipur Board Class 10 Nepali
- Rajasthan Board Class 11 Informatics Practices
- Rajasthan Board Class 10 English Core
- Madhya Pradesh Board Class 9 Arabic
- ICSE Board Class 10 Computer Science
- Madhya Pradesh Board Class 12 Biology
- Gujarat Board Class 12 English Core
- Himachal Pradesh Board Class 11 Urdu Core
- Madhya Pradesh Board Class 12 Gujarati (special)
- Himachal Pradesh Board Class 10 Art


